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A DNA unwinding element (DUE or DNAUE) is the initiation site for the opening of the double helix structure of the DNA at the origin of replication for DNA synthesis. It is A-T rich and denatures easily due to its low helical stability, which allows the single-strand region to be recognized by origin recognition complex. DUEs are found in both prokaryotic and eukaryotic organisms, but were first discovered in yeast and bacteria origins, by Huang Kowalski. The DNA unwinding allows for access of replication machinery to the newly single strands. In eukaryotes, DUEs are the binding site for DNA-unwinding element binding (DUE-B) proteins required for replication initiation. In prokaryotes, DUEs are found in the form of tandem consensus sequences flanking the 5' e… more »

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My cup so dark MLK had a dream
My bro wear a mask like it's COVID-19
I'm high everyday but it's not what it seems
I know that boy broke he ain't higher than me
Yeah, I got the method like Davis McLean
She hate when I sip, told me stay off the lean
Fell asleep while driving almost fucked up the V
My rap name Chip all these bitches chase me
My bitch hate me cause I still chase lean
But what can I say I'm a rich junkie
Bro where you gon' go if the Glock got a beam
Leaning hard off drink like my bro push me
Should've knew he a bitch boy bump Toosii
I was geeked all night like I took 2C
Pockets fatter than ever take a dip blue cheese
Heart beat out my chest boy I love ecstasy
I'm rapping so low cause I'm high as giraffe
Boy, you gon' die if I go call bash
Bro Stay with the strap like you're rocking a sash
Sip lean move slow but I still drive fast
That boy got robbed cause he flashing his cash
All this cash in my pocket just making me sag
Niggas really be bitches everything is a drag
I buy what I want I don't care bout a tag
I sip everyday I don't care if I lag
65 in the drake like I just passed class
Bro just lit the P.A.I.N thought that boy passed gas
Don't touch my cup boy it just got stabbed
Nigga still want smoke but his man's got stabbed
She think I'm laced everyday I spazz
The inside brown we spin like tazz
I'm nodding off, off the sip I had
If I fall asleep wake me up my bad
I love your bitch why she so bad
I'm balling hard you tore your calf
You broke no joke can't help but laugh
Bro play with sticks like he got staff
I know he cap we never asked
Yea I got blue like handicap nigga
What the f*ck
Nigga so cap that nigga so cap I ain't never ask that nigga nothing the f*ck he talking to us

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