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Elisa

The enzyme-linked immunosorbent assay (ELISA) (, ) is a commonly used analytical biochemistry assay, first described by Eva Engvall and Peter Perlmann in 1971. The assay uses a solid-phase type of enzyme immunoassay (EIA) to detect the presence of a ligand (commonly a protein) in a liquid sample using antibodies directed against the protein to be measured. ELISA has been used as a diagnostic tool in medicine, plant pathology, and biotechnology, as well as a quality control check in various industries. In the most simple form of an ELISA, antigens from the sample to be tested are attached to a surface. Then, a matching antibody is applied over the surface so it can bind the antigen. This antibody is linked to an enzyme and then any unbound antibodies are remov… more »

Year:

2007

4:11

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You did not dare say a single word
I did not dare ask for something more
I've kept my questions secret deep inside
But I wish I could have let you know about
A time when I would have said

Wait, and please stay
Did you mean to push me away?
Please wait and just stay
Did you want it to be this way?

Would you want to know what I've been through?
(Through all this time, all this time)
Would you want to know I have missed you too
(And I have you on my mind)
And you've been and you will be a part of me
(That I can't find)
And you've been forgiven for your silence
All this time when I would have said

Wait, and please stay
Did you mean to push me away?
Please wait and just say
Is there a way that could replace
The times you never said
How've you been?
Do you need anything?
Want you to know I'm here?
Want you to feel me near?

Yeah and I hope
I hope that you will find your way
Yeah and I hope
I hope there will be better days

Please wait, and just stay
(Please stay)
Did you mean to push me away?
Please wait, and just stay
(Wait, just stay)
Did you want it to be this way?
Wait, please say
(Yeah, and I hope)
I hope that you will find your way
Please wait, and just stay
(Wait, and I hope)
I hope there will be better days

Hey hey

Wait
Please stay
Just stay
Please wait

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