The Big Dipper

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Elisa

The enzyme-linked immunosorbent assay (ELISA) (, ) is a commonly used analytical biochemistry assay, first described by Eva Engvall and Peter Perlmann in 1971. The assay uses a solid-phase type of enzyme immunoassay (EIA) to detect the presence of a ligand (commonly a protein) in a liquid sample using antibodies directed against the protein to be measured. ELISA has been used as a diagnostic tool in medicine, plant pathology, and biotechnology, as well as a quality control check in various industries. In the most simple form of an ELISA, antigens from the sample to be tested are attached to a surface. Then, a matching antibody is applied over the surface so it can bind the antigen. This antibody is linked to an enzyme and then any unbound antibodies are remov… more »

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When there's no light I can follow
I look at the sky
I look at the big dipper
Have you got a place? 
A tiny little one would do
For when I can't walk and I cannot make it on my own

'Cause I have so much fire here I have much to burn
But could I come closer if I were cold?

Don't say no 
To your daughter
A flower
Someone swimming against the current 
To a, to a, to a, to a, hey

When everything gets hollow 
I look at the sky
I look at the big dipper
Is there any space? 
Could you make some room for me? 
For when I feel small, and I don't know anything at all

Well, I have so much fire here I have much to burn
But could I come closer if I were cold

Don't say no
To your daughter
To a, a fighter 
To a, a stranger 
To a, a believer
To a, a flower 
To a, a loser 
To a, a mother 
To a, a prayer
Don't say no
To a daughter
To a, a fighter 
To a, a stranger 
To a, a believer
To a, a flower 
To a, a loser 
To a, a mother 
To a, a prayer

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